Precursor of Egg White Lysozyme
نویسندگان
چکیده
Lysozyme mRNA was translated in a reticulocyte lysate with mixtures of radioactive amino acids. The in vitro product isolated by immunoprecipitation was shown by gel electrophoresis, peptide mapping, and sequence analysis to be larger than lysozyme synthesized in uiuo. An NH,-terminal extension was completely sequenced by automated Edman degradation; the phenylthiohydantoins from each cycle were separated by high pressure liquid chromatography and quantitated by scintillation spectroscopy. The NH,-terminal sequence of pre-lysozyme is:
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تاریخ انتشار 2002